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fdh1 mcherry fusion  (Addgene inc)


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    Addgene inc fdh1 mcherry fusion
    Localization of Arabidopsis formate dehydrogenase 1. (a) and (b) Arabidopsis formate dehydrogenase 1 (FDH1) was C-terminally fused with mCherry. Transgenic Arabidopsis lines <t>expressing</t> <t>FDH1-mCherry</t> under the constitutive UBQ10 promoter were generated. Leaves (epidermal cell layer, (a) and roots (root cortex, (b) of 14-d-old seedlings were analyzed by confocal laser scanning microscopy. Red = FDH1-mCherry signal, Blue = MitoTracker Green FM as mitochondrial marker, in (a) Cyan = chlorophyll A autofluorescence. (c) FDH activity of FDH1-mCherry fusion. FDH1-mCherry was heterologously expressed in E. coli. The respective lysate was measured for FDH activity and compared to the empty vector control (EV). n = 3, Shown = mean ± SD, replicates shown as points. (d) The tissue-specific expression of FDH1, in seedlings at 5, 7, and 15 d after germination was analyzed by FDH1p:GUS fusions . The asterisk marks the transition from elongation to differentiation zone.
    Fdh1 Mcherry Fusion, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fdh1+mcherry+fusion/pICH86966+(Plasmid+%2346967)/pmc12854405-198-1-7
    Average 93 stars, based on 12 article reviews
    fdh1 mcherry fusion - by Bioz Stars, 2026-10
    93/100 stars

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    1) Product Images from "Mitochondrial formate dehydrogenase links mitochondrial and cytosolic one carbon metabolism via a one-carbon shunt"

    Article Title: Mitochondrial formate dehydrogenase links mitochondrial and cytosolic one carbon metabolism via a one-carbon shunt

    Journal: Plant Physiology

    doi: 10.1093/plphys/kiaf623

    Localization of Arabidopsis formate dehydrogenase 1. (a) and (b) Arabidopsis formate dehydrogenase 1 (FDH1) was C-terminally fused with mCherry. Transgenic Arabidopsis lines expressing FDH1-mCherry under the constitutive UBQ10 promoter were generated. Leaves (epidermal cell layer, (a) and roots (root cortex, (b) of 14-d-old seedlings were analyzed by confocal laser scanning microscopy. Red = FDH1-mCherry signal, Blue = MitoTracker Green FM as mitochondrial marker, in (a) Cyan = chlorophyll A autofluorescence. (c) FDH activity of FDH1-mCherry fusion. FDH1-mCherry was heterologously expressed in E. coli. The respective lysate was measured for FDH activity and compared to the empty vector control (EV). n = 3, Shown = mean ± SD, replicates shown as points. (d) The tissue-specific expression of FDH1, in seedlings at 5, 7, and 15 d after germination was analyzed by FDH1p:GUS fusions . The asterisk marks the transition from elongation to differentiation zone.
    Figure Legend Snippet: Localization of Arabidopsis formate dehydrogenase 1. (a) and (b) Arabidopsis formate dehydrogenase 1 (FDH1) was C-terminally fused with mCherry. Transgenic Arabidopsis lines expressing FDH1-mCherry under the constitutive UBQ10 promoter were generated. Leaves (epidermal cell layer, (a) and roots (root cortex, (b) of 14-d-old seedlings were analyzed by confocal laser scanning microscopy. Red = FDH1-mCherry signal, Blue = MitoTracker Green FM as mitochondrial marker, in (a) Cyan = chlorophyll A autofluorescence. (c) FDH activity of FDH1-mCherry fusion. FDH1-mCherry was heterologously expressed in E. coli. The respective lysate was measured for FDH activity and compared to the empty vector control (EV). n = 3, Shown = mean ± SD, replicates shown as points. (d) The tissue-specific expression of FDH1, in seedlings at 5, 7, and 15 d after germination was analyzed by FDH1p:GUS fusions . The asterisk marks the transition from elongation to differentiation zone.

    Techniques Used: Transgenic Assay, Expressing, Generated, Confocal Laser Scanning Microscopy, Marker, Activity Assay, Plasmid Preparation, Control

    Related Articles

    Transgenic Assay:

    Article Title: Mitochondrial formate dehydrogenase links mitochondrial and cytosolic one carbon metabolism via a one-carbon shunt
    Article Snippet: BsaI and BpiI restriction sites of the FDH1 coding sequence were removed by gene synthesis (ThermoFisher Scientific, Waltham, USA). .. The FDH1-mCherry fusion was assembled in pICH86966 (Addgene #46967). .. Single-guide RNA sites and plasmids for targeted mutagenesis of the FDH1 gene by CRISPR/Cas9 were designed and cloned as described in ( ).

    Article Title: Mitochondrial formate dehydrogenase links mitochondrial and cytosolic one carbon metabolism via a one-carbon shunt.
    Article Snippet: BsaI and BpiI restriction sites of the 28 FDH1 coding sequence were removed by gene synthesis (ThermoFisher Scientific, Waltham, 29 USA). .. The FDH1-mCherry fusion was assembled in pICH86966 (Addgene #46967). .. Single guide 30 RNA sites and plasmids for targeted mutagenesis of the FDH1 gene by CRISPR/Cas9 were 31 AC CE PT ED M AN US CR IP T nloaded from h

    Expressing:

    Article Title: Mitochondrial formate dehydrogenase links mitochondrial and cytosolic one carbon metabolism via a one-carbon shunt
    Article Snippet: BsaI and BpiI restriction sites of the FDH1 coding sequence were removed by gene synthesis (ThermoFisher Scientific, Waltham, USA). .. The FDH1-mCherry fusion was assembled in pICH86966 (Addgene #46967). .. Single-guide RNA sites and plasmids for targeted mutagenesis of the FDH1 gene by CRISPR/Cas9 were designed and cloned as described in ( ).

    Article Title: Mitochondrial formate dehydrogenase links mitochondrial and cytosolic one carbon metabolism via a one-carbon shunt.
    Article Snippet: BsaI and BpiI restriction sites of the 28 FDH1 coding sequence were removed by gene synthesis (ThermoFisher Scientific, Waltham, 29 USA). .. The FDH1-mCherry fusion was assembled in pICH86966 (Addgene #46967). .. Single guide 30 RNA sites and plasmids for targeted mutagenesis of the FDH1 gene by CRISPR/Cas9 were 31 AC CE PT ED M AN US CR IP T nloaded from h

    Generated:

    Article Title: Mitochondrial formate dehydrogenase links mitochondrial and cytosolic one carbon metabolism via a one-carbon shunt
    Article Snippet: BsaI and BpiI restriction sites of the FDH1 coding sequence were removed by gene synthesis (ThermoFisher Scientific, Waltham, USA). .. The FDH1-mCherry fusion was assembled in pICH86966 (Addgene #46967). .. Single-guide RNA sites and plasmids for targeted mutagenesis of the FDH1 gene by CRISPR/Cas9 were designed and cloned as described in ( ).

    Article Title: Mitochondrial formate dehydrogenase links mitochondrial and cytosolic one carbon metabolism via a one-carbon shunt.
    Article Snippet: BsaI and BpiI restriction sites of the 28 FDH1 coding sequence were removed by gene synthesis (ThermoFisher Scientific, Waltham, 29 USA). .. The FDH1-mCherry fusion was assembled in pICH86966 (Addgene #46967). .. Single guide 30 RNA sites and plasmids for targeted mutagenesis of the FDH1 gene by CRISPR/Cas9 were 31 AC CE PT ED M AN US CR IP T nloaded from h

    Confocal Laser Scanning Microscopy:

    Article Title: Mitochondrial formate dehydrogenase links mitochondrial and cytosolic one carbon metabolism via a one-carbon shunt
    Article Snippet: BsaI and BpiI restriction sites of the FDH1 coding sequence were removed by gene synthesis (ThermoFisher Scientific, Waltham, USA). .. The FDH1-mCherry fusion was assembled in pICH86966 (Addgene #46967). .. Single-guide RNA sites and plasmids for targeted mutagenesis of the FDH1 gene by CRISPR/Cas9 were designed and cloned as described in ( ).

    Article Title: Mitochondrial formate dehydrogenase links mitochondrial and cytosolic one carbon metabolism via a one-carbon shunt.
    Article Snippet: BsaI and BpiI restriction sites of the 28 FDH1 coding sequence were removed by gene synthesis (ThermoFisher Scientific, Waltham, 29 USA). .. The FDH1-mCherry fusion was assembled in pICH86966 (Addgene #46967). .. Single guide 30 RNA sites and plasmids for targeted mutagenesis of the FDH1 gene by CRISPR/Cas9 were 31 AC CE PT ED M AN US CR IP T nloaded from h

    Marker:

    Article Title: Mitochondrial formate dehydrogenase links mitochondrial and cytosolic one carbon metabolism via a one-carbon shunt
    Article Snippet: BsaI and BpiI restriction sites of the FDH1 coding sequence were removed by gene synthesis (ThermoFisher Scientific, Waltham, USA). .. The FDH1-mCherry fusion was assembled in pICH86966 (Addgene #46967). .. Single-guide RNA sites and plasmids for targeted mutagenesis of the FDH1 gene by CRISPR/Cas9 were designed and cloned as described in ( ).

    Article Title: Mitochondrial formate dehydrogenase links mitochondrial and cytosolic one carbon metabolism via a one-carbon shunt.
    Article Snippet: BsaI and BpiI restriction sites of the 28 FDH1 coding sequence were removed by gene synthesis (ThermoFisher Scientific, Waltham, 29 USA). .. The FDH1-mCherry fusion was assembled in pICH86966 (Addgene #46967). .. Single guide 30 RNA sites and plasmids for targeted mutagenesis of the FDH1 gene by CRISPR/Cas9 were 31 AC CE PT ED M AN US CR IP T nloaded from h

    Activity Assay:

    Article Title: Mitochondrial formate dehydrogenase links mitochondrial and cytosolic one carbon metabolism via a one-carbon shunt
    Article Snippet: BsaI and BpiI restriction sites of the FDH1 coding sequence were removed by gene synthesis (ThermoFisher Scientific, Waltham, USA). .. The FDH1-mCherry fusion was assembled in pICH86966 (Addgene #46967). .. Single-guide RNA sites and plasmids for targeted mutagenesis of the FDH1 gene by CRISPR/Cas9 were designed and cloned as described in ( ).

    Article Title: Mitochondrial formate dehydrogenase links mitochondrial and cytosolic one carbon metabolism via a one-carbon shunt.
    Article Snippet: BsaI and BpiI restriction sites of the 28 FDH1 coding sequence were removed by gene synthesis (ThermoFisher Scientific, Waltham, 29 USA). .. The FDH1-mCherry fusion was assembled in pICH86966 (Addgene #46967). .. Single guide 30 RNA sites and plasmids for targeted mutagenesis of the FDH1 gene by CRISPR/Cas9 were 31 AC CE PT ED M AN US CR IP T nloaded from h

    Plasmid Preparation:

    Article Title: Mitochondrial formate dehydrogenase links mitochondrial and cytosolic one carbon metabolism via a one-carbon shunt
    Article Snippet: BsaI and BpiI restriction sites of the FDH1 coding sequence were removed by gene synthesis (ThermoFisher Scientific, Waltham, USA). .. The FDH1-mCherry fusion was assembled in pICH86966 (Addgene #46967). .. Single-guide RNA sites and plasmids for targeted mutagenesis of the FDH1 gene by CRISPR/Cas9 were designed and cloned as described in ( ).

    Article Title: Mitochondrial formate dehydrogenase links mitochondrial and cytosolic one carbon metabolism via a one-carbon shunt.
    Article Snippet: BsaI and BpiI restriction sites of the 28 FDH1 coding sequence were removed by gene synthesis (ThermoFisher Scientific, Waltham, 29 USA). .. The FDH1-mCherry fusion was assembled in pICH86966 (Addgene #46967). .. Single guide 30 RNA sites and plasmids for targeted mutagenesis of the FDH1 gene by CRISPR/Cas9 were 31 AC CE PT ED M AN US CR IP T nloaded from h

    Control:

    Article Title: Mitochondrial formate dehydrogenase links mitochondrial and cytosolic one carbon metabolism via a one-carbon shunt
    Article Snippet: BsaI and BpiI restriction sites of the FDH1 coding sequence were removed by gene synthesis (ThermoFisher Scientific, Waltham, USA). .. The FDH1-mCherry fusion was assembled in pICH86966 (Addgene #46967). .. Single-guide RNA sites and plasmids for targeted mutagenesis of the FDH1 gene by CRISPR/Cas9 were designed and cloned as described in ( ).

    Article Title: Mitochondrial formate dehydrogenase links mitochondrial and cytosolic one carbon metabolism via a one-carbon shunt.
    Article Snippet: BsaI and BpiI restriction sites of the 28 FDH1 coding sequence were removed by gene synthesis (ThermoFisher Scientific, Waltham, 29 USA). .. The FDH1-mCherry fusion was assembled in pICH86966 (Addgene #46967). .. Single guide 30 RNA sites and plasmids for targeted mutagenesis of the FDH1 gene by CRISPR/Cas9 were 31 AC CE PT ED M AN US CR IP T nloaded from h



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    fdh1 mcherry fusion  (Addgene inc)
    93
    Addgene inc fdh1 mcherry fusion
    Localization of Arabidopsis formate dehydrogenase 1. (a) and (b) Arabidopsis formate dehydrogenase 1 (FDH1) was C-terminally fused with mCherry. Transgenic Arabidopsis lines <t>expressing</t> <t>FDH1-mCherry</t> under the constitutive UBQ10 promoter were generated. Leaves (epidermal cell layer, (a) and roots (root cortex, (b) of 14-d-old seedlings were analyzed by confocal laser scanning microscopy. Red = FDH1-mCherry signal, Blue = MitoTracker Green FM as mitochondrial marker, in (a) Cyan = chlorophyll A autofluorescence. (c) FDH activity of FDH1-mCherry fusion. FDH1-mCherry was heterologously expressed in E. coli. The respective lysate was measured for FDH activity and compared to the empty vector control (EV). n = 3, Shown = mean ± SD, replicates shown as points. (d) The tissue-specific expression of FDH1, in seedlings at 5, 7, and 15 d after germination was analyzed by FDH1p:GUS fusions . The asterisk marks the transition from elongation to differentiation zone.
    Fdh1 Mcherry Fusion, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/fdh1+mcherry+fusion/pICH86966+(Plasmid+%2346967)/pmc12854405-198-1-7
    Average 93 stars, based on 1 article reviews
    fdh1 mcherry fusion - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Localization of Arabidopsis formate dehydrogenase 1. (a) and (b) Arabidopsis formate dehydrogenase 1 (FDH1) was C-terminally fused with mCherry. Transgenic Arabidopsis lines expressing FDH1-mCherry under the constitutive UBQ10 promoter were generated. Leaves (epidermal cell layer, (a) and roots (root cortex, (b) of 14-d-old seedlings were analyzed by confocal laser scanning microscopy. Red = FDH1-mCherry signal, Blue = MitoTracker Green FM as mitochondrial marker, in (a) Cyan = chlorophyll A autofluorescence. (c) FDH activity of FDH1-mCherry fusion. FDH1-mCherry was heterologously expressed in E. coli. The respective lysate was measured for FDH activity and compared to the empty vector control (EV). n = 3, Shown = mean ± SD, replicates shown as points. (d) The tissue-specific expression of FDH1, in seedlings at 5, 7, and 15 d after germination was analyzed by FDH1p:GUS fusions . The asterisk marks the transition from elongation to differentiation zone.

    Journal: Plant Physiology

    Article Title: Mitochondrial formate dehydrogenase links mitochondrial and cytosolic one carbon metabolism via a one-carbon shunt

    doi: 10.1093/plphys/kiaf623

    Figure Lengend Snippet: Localization of Arabidopsis formate dehydrogenase 1. (a) and (b) Arabidopsis formate dehydrogenase 1 (FDH1) was C-terminally fused with mCherry. Transgenic Arabidopsis lines expressing FDH1-mCherry under the constitutive UBQ10 promoter were generated. Leaves (epidermal cell layer, (a) and roots (root cortex, (b) of 14-d-old seedlings were analyzed by confocal laser scanning microscopy. Red = FDH1-mCherry signal, Blue = MitoTracker Green FM as mitochondrial marker, in (a) Cyan = chlorophyll A autofluorescence. (c) FDH activity of FDH1-mCherry fusion. FDH1-mCherry was heterologously expressed in E. coli. The respective lysate was measured for FDH activity and compared to the empty vector control (EV). n = 3, Shown = mean ± SD, replicates shown as points. (d) The tissue-specific expression of FDH1, in seedlings at 5, 7, and 15 d after germination was analyzed by FDH1p:GUS fusions . The asterisk marks the transition from elongation to differentiation zone.

    Article Snippet: The FDH1-mCherry fusion was assembled in pICH86966 (Addgene #46967).

    Techniques: Transgenic Assay, Expressing, Generated, Confocal Laser Scanning Microscopy, Marker, Activity Assay, Plasmid Preparation, Control